# q2-itsxpress creates incomplete manifest after trim-pair-output-unmerged

**URL:** https://forum.qiime2.org/t/q2-itsxpress-creates-incomplete-manifest-after-trim-pair-output-unmerged/10243
**Category:** Library Support
**Tags:** dada2, its, fungi
**Created:** [June 14, 2019, 1:08pm UTC](https://forum.qiime2.org/t/q2-itsxpress-creates-incomplete-manifest-after-trim-pair-output-unmerged/10243 "2019-06-14T13:08:42Z")
**Posts on this page:** 4
**Page:** 1

<div class="post-metadata">

### Author: ![etmmaas](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/e/ac91a4/32.png) [@etmmaas](https://forum.qiime2.org/u/etmmaas)
#### Post date: [June 14, 2019, 1:08pm UTC](https://forum.qiime2.org/t/q2-itsxpress-creates-incomplete-manifest-after-trim-pair-output-unmerged/10243/1 "2019-06-14T13:08:42Z")

</div>

Hi,

I have been using q2-itsxpress to extract the its2 region before running dada2. The qiime itsxpress trim-pair-output-unmerged seems to work fine, but the output gives .qza files where the manifest file only contains information for the forward sequences, [as discussed in this topic](https://forum.qiime2.org/t/merging-or-not-its2-amplicons-with-variable-length/7868). So I was wondering if there has been some work to fix this.

 ![manifest](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/2X/f/fa9f5b22940571770580fe43b3dcce8620591fb9.png)

---

<div class="post-metadata">

### Author: ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [June 14, 2019, 2:37pm UTC](https://forum.qiime2.org/t/q2-itsxpress-creates-incomplete-manifest-after-trim-pair-output-unmerged/10243/2 "2019-06-14T14:37:38Z")

</div>

Thank you for reporting @etmmaas! Let's see what @Adam_Rivers has to say about this.

---

<div class="post-metadata">

### Author: ![Adam\_Rivers](https://forum.qiime2.org/user_avatar/forum.qiime2.org/adam_rivers/32/10143_2.png) [@Adam\_Rivers](https://forum.qiime2.org/u/Adam_Rivers)
#### Post date: [June 14, 2019, 7:46pm UTC](https://forum.qiime2.org/t/q2-itsxpress-creates-incomplete-manifest-after-trim-pair-output-unmerged/10243/3 "2019-06-14T19:46:02Z")

</div>

I was able to replicate this issue, but it was fixed by version 1.7.3 available on Github March 8, 2019.

Unfortunately, I was on the wrong branch when I created the the wheel for version 1.7.3 on Pip. So Pip still has the old version 1.7.2 masquerading as 1.7.3.

I will (carefully) increase the version to 1.7.4 on both Github and pip and let you know. Then you should be able to upgrade with pip.

I need to switch to a better versioning system, maybe Versioneer...

---

<div class="post-metadata">

### Author: ![Adam\_Rivers](https://forum.qiime2.org/user_avatar/forum.qiime2.org/adam_rivers/32/10143_2.png) [@Adam\_Rivers](https://forum.qiime2.org/u/Adam_Rivers)
#### Post date: [June 14, 2019, 8:28pm UTC](https://forum.qiime2.org/t/q2-itsxpress-creates-incomplete-manifest-after-trim-pair-output-unmerged/10243/4 "2019-06-14T20:28:15Z")

</div>

Okay, upgrading the Qiime 2 plugin `q2_itsxpress` to v1.7.4 using pip should solve this now:

```auto
pip install update q2_itsxpress

```

You should also verify that the stand-alone program `itsxpress` is up to date too (v 1.7.2)

```auto
conda update itsxpress

```