# Q2-ITSxpress: A tutorial on a QIIME 2 plugin to trim ITS sequences

**URL:** https://forum.qiime2.org/t/q2-itsxpress-a-tutorial-on-a-qiime-2-plugin-to-trim-its-sequences/5780
**Category:** Plugins
**Tags:** its, fungi, tutorial
**Created:** [August 27, 2018, 7:11pm UTC](https://forum.qiime2.org/t/q2-itsxpress-a-tutorial-on-a-qiime-2-plugin-to-trim-its-sequences/5780 "2018-08-27T19:11:02Z")
**Posts on this page:** 1
**Showing post:** 9

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### Author: ![thermokarst](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/t/8e7dd6/32.png) [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [August 28, 2018, 4:39pm UTC](https://forum.qiime2.org/t/q2-itsxpress-a-tutorial-on-a-qiime-2-plugin-to-trim-its-sequences/5780/9 "2018-08-28T16:39:58Z")

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@Lorinda --- I am not involved with the development of this plugin, but it looks like you are trying to use _multiplexed_ sequences where _demultiplexed_ sequences are expected.

Use `demux emp-paired` to demux, then provide that is input to `itsexpress`.

> [@Lorinda](#):
>
> Is this something that this plugin will accept in the future?

Again, I am not involved with the development of this plugin, but I would suggest that maybe that kind of functionality doesn't make sense in the context of this plugin. I think the general design idea here in QIIME 2 is to compose modular building blocks of functionality. Each plugin/method should do one thing, and one thing well.

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