Hi @Hayley_Guay ,
The key line in your error is here:
Sample 1 has an invalid maximum Phred Quality Scores of 131097
We've seen this error before when an arbitrarily edited quality score was used or if the sequencing technology somehow is assigning q-scores not within the Phred 33 schema. Have you seen this thread for some suggestions with a similar problem? If not, have a look there first and let us know if you're still stuck. Thanks!