# q2-dada2 and NextSeq amplicon data

**URL:** https://forum.qiime2.org/t/q2-dada2-and-nextseq-amplicon-data/29393
**Category:** General Discussion
**Tags:** dada2
**Created:** [February 26, 2024, 3:05pm UTC](https://forum.qiime2.org/t/q2-dada2-and-nextseq-amplicon-data/29393 "2024-02-26T15:05:24Z")
**Posts on this page:** 1
**Showing post:** 2

<div class="post-metadata">

### Author: ![buzic](https://forum.qiime2.org/user_avatar/forum.qiime2.org/buzic/32/16494_2.png) [@buzic](https://forum.qiime2.org/u/buzic)
#### Post date: [February 26, 2024, 4:30pm UTC](https://forum.qiime2.org/t/q2-dada2-and-nextseq-amplicon-data/29393/2 "2024-02-26T16:30:39Z")

</div>

HI @ctekellogg 👋

I think the NextSeq and the NovaSeq have the same quality binning and 2 colour chemisty. So what applies to one would apply to the other regarding denoising. There is a really good breakdown of NovaSeq processing issues [here](https://forum.qiime2.org/t/novaseq-and-dada2-incompatibility/25865). I hope thats helpful.

Basically, I don't think you can't use dada2 - but you are correct in that it has some issues associated with it. I am unsure if the development team of Qiime2 have implimented anything along these lines.

Best,

Vic 😃

---

_[View the full topic](https://forum.qiime2.org/t/q2-dada2-and-nextseq-amplicon-data/29393)._