Hello out there!
I do work with Qiime2 via Virtualbox and the Version 2020.2.
When I do deblur, I get this message
(qiime2-2020.2) qiime2@qiime2core2020-2:~/Desktop/data$ qiime deblur denoise-16S --i-demultiplexed-seqs demux-filtered.qza --p-trim-length 240 --o-representative-sequences representative_sequences.qza --o-table table.qza --p-sample-stats --o-stats denoising-stats.qza
Plugin error from deblur:
Command ‘[‘deblur’, ‘workflow’, ‘–seqs-fp’, ‘/tmp/qiime2-archive-lwvh1a6h/827319bb-8ae8-4a2f-b043-6490b4b5955e/data’, ‘–output-dir’, ‘/tmp/tmpi47927vs’, ‘–mean-error’, ‘0.005’, ‘–indel-prob’, ‘0.01’, ‘–indel-max’, ‘3’, ‘–trim-length’, ‘240’, ‘–left-trim-length’, ‘0’, ‘–min-reads’, ‘10’, ‘–min-size’, ‘2’, ‘–jobs-to-start’, ‘1’, ‘-w’, ‘–keep-tmp-files’]’ returned non-zero exit status 1.
Debug info has been saved to /tmp/qiime2-q2cli-err-y8jeml9d.log
I made sure, that I installed deblur properly.
Everything works, til I add the line --o-stats denoising-stats.qza to (qiime2-2020.2) qiime2@qiime2core2020-2:~/Desktop/data$ qiime deblur denoise-16S --i-demultiplexed-seqs demux-filtered.qza --p-trim-length 240 --o-representative-sequences representative_sequences.qza --o-table table.qza --p-sample-stats
Me and my boss, were not able to fix the problem or find the reason.
Please help! 