Dear Qiime developers,
When I run dada2 with the following code in the terminal, something was wrong. I want to leave the 0-195 fragments and cut off the bases after 195. However, when visualizing rep-seq.qzv, the intercepted fragments are not correct.
time qiime dada2 denoise-paired
–i-demultiplexed-seqs paired-end-demux.qza
–p-trim-left-f 0
–p-trim-left-r 0
–p-trunc-len-f 195
–p-trunc-len-r 195
–o-table table.qza
–o-representative-sequences rep-seqs.qza
–o-denoising-stats dada2-stats.qza \
Do you have any idea how to solve this issue ?
Thanks a ton