I'm new working with Illumina reads. When I visualize my demux-paired-end.qzv file I obtain the following image:
If we focus on, for example, the forward reads graph the bars between positions 20 and 100 are extremely short. You can see it if I amplify the image:
If I amplify the image more, in position 25 for example, you can see it better:
How I should interpretate the extremely short bars? Is it normal working with Illumina reads? Should I carry on with my analysis assuming that the sequences are OK?
Thank you all,