Problem with importing single end files


I want to analyze microbiome data and, at import moment, I cannot solve the error that appears:

There was a problem importing /home/talita/exp15/manifest:

Missing one or more files for SingleLanePerSampleSingleEndFastqDirFmt: '.+_.+_L[0-9][0-9][0-9]_R[12]_001\.fastq\.gz'

I've already tried to find any answer in the forum but without success.

Obs: talita is the user name; exp15 is the folders name; I have single end fastq unzipped data.

I appreciate for any help

Best regards,


Can you copy and paste the command you used to attempt to import your files here? And your manifest file too? It looks like QIIME 2 is having trouble finding your files, so the output of an ls command on the directory you are trying to run import from might be helpful too :slightly_smiling_face:

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