Hello there, i have issue importing my fastq files using a manifest file i created, after i run the the code i encountered the following error:
(qiime2-2022.2) qiime2@qiime2core2022-2:/media/sf_Qiime2$ qiime tools import \
--input-path manifest.csv
--type 'SampleData[SequencesWithQuality]'
--input-format SingleEndFastqManifestPhred33V2
--output-path se-demux.qza
Traceback (most recent call last):
File "/home/qiime2/miniconda/envs/qiime2-2022.2/lib/python3.8/site-packages/q2cli/builtin/tools.py", line 157, in import_data
artifact = qiime2.sdk.Artifact.import_data(type, input_path,
File "/home/qiime2/miniconda/envs/qiime2-2022.2/lib/python3.8/site-packages/qiime2/sdk/result.py", line 277, in import_data
return cls.from_view(type, view, view_type, provenance_capture,
File "/home/qiime2/miniconda/envs/qiime2-2022.2/lib/python3.8/site-packages/qiime2/sdk/result.py", line 305, in _from_view
result = transformation(view, validate_level)
File "/home/qiime2/miniconda/envs/qiime2-2022.2/lib/python3.8/site-packages/qiime2/core/transform.py", line 68, in transformation
self.validate(view, level=validate_level)
File "/home/qiime2/miniconda/envs/qiime2-2022.2/lib/python3.8/site-packages/qiime2/core/transform.py", line 143, in validate
view.validate(level)
File "/home/qiime2/miniconda/envs/qiime2-2022.2/lib/python3.8/site-packages/qiime2/plugin/model/file_format.py", line 26, in validate
self.validate(level)
File "/home/qiime2/miniconda/envs/qiime2-2022.2/lib/python3.8/site-packages/q2_types/per_sample_sequences/_format.py", line 40, in validate
md = qiime2.Metadata.load(str(self))
File "/home/qiime2/miniconda/envs/qiime2-2022.2/lib/python3.8/site-packages/qiime2/metadata/metadata.py", line 396, in load
return MetadataReader(filepath).read(
File "/home/qiime2/miniconda/envs/qiime2-2022.2/lib/python3.8/site-packages/qiime2/metadata/io.py", line 73, in read
header = self._read_header()
File "/home/qiime2/miniconda/envs/qiime2-2022.2/lib/python3.8/site-packages/qiime2/metadata/io.py", line 144, in _read_header
for row in self._reader:
File "/home/qiime2/miniconda/envs/qiime2-2022.2/lib/python3.8/site-packages/qiime2/metadata/io.py", line 71, in
self._reader = (self._strip_cell_whitespace(row)
_csv.Error: ' ' expected after '"'
Thank you very much it was really helpful, however i encountred another problem after importing the data when i tried to visualize the sequence reads as follows
qiime2-2022.2) qiime2@qiime2core2022-2:/media/sf_Qiime2$ qiime tools import \
--type 'SampleData[SequencesWithQuality]'
--input-path manifest2.txt
--output-path single-end-demux.qza
--input-format SingleEndFastqManifestPhred33V2
Imported manifest2.txt as SingleEndFastqManifestPhred33V2 to single-end-demux.qza
(qiime2-2022.2) qiime2@qiime2core2022-2:/media/sf_Qiime2$ qiime demux summarize
--i-data single-end-demux.qza
--o-visualization se-demux.qzv
Plugin error from demux:
'utf-8' codec can't decode byte 0xc6 in position 4227: invalid continuation byte
Debug info has been saved to /tmp/qiime2-q2cli-err-jlzu50sn.log