I have dada2 rep-seqs-dada2.qza file and wanted to do taxonomic analysis. Because of memory issues with my laptop, I have to train my classifier using my institute HPC server. When I import my rep-seqs-dada2.qza into qiime2 in the HPC server using the following commands:
qiime tools import
It gave following error:
clsuterdata/uqmtaman/scratch/Analysis/rep-seqs-dada2.qza is no a(an) DNAFASTAFormat file: First line of the file is not a valid description. Descriptions must start with '>'.
Could you help me with this or guide me to any forum discussion if any.
Hi @Man - that file already appears to be QIIME 2 Artifact, which means does not need to be imported. You should be good to proceed with using that file in your downstream steps of your analysis.
Thanks Matt. I was thinking that way because I am now switching to the institute HPC server for further analysis. Also, the institute HPC server did not have qiime2-2021.4 version and the IT guys helped me install the software on the server.
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