Dear all, I am analysing fungi data obtained from a capillery sequencer. I have converted the fasta file to fastq files using seqtk. After loading them into QIIME2 using the type paired end sequencing and the result I am getting from the qzv file is only 1 sequence per sample. So I separated the f…

When I was speaking of the quality scores, I was referring to using DADA2. As you mentioned you were going to use... Denoising tools, like DADA2 require FASTQ files. [image] sree: If there is anyway I can simply import and use the fasta files and do the alignment and taxonomy mapping and furth…

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Problem with capillery FASTA data handling

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