Hi!
I am trying to run ALDEx2 in qiime2-2020.2 version. But I get this error:
Plugin error from aldex2:
** An error was encountered while running ALDEx2 in R (return code 1), please inspect stdout and stderr to learn more.**
Debug info has been saved to /tmp/qiime2-q2cli-err-31aztdjt.log
log file:
Running external command line application(s). This may print messages to stdout and/or stderr.
The command(s) being run are below. These commands cannot be manually re-run as they will depend on temporary files that no longer exist.
Command: run_aldex2.R /tmp/tmpiwex_o1o/input.tsv.biom /tmp/tmpiwex_o1o/input.map.txt sample 128 t all /tmp/tmpiwex_o1o/output.summary.txt
R version 3.5.1 (2018-07-02)
[1] âaldex.clr: generating Monte-Carlo instances and clr valuesâ
[1] âoperating in serial modeâ
[1] âcomputing center with all featuresâ
[1] âaldex.ttest: doing t-testâ
Error in aldex.ttest(x, conditions, paired.test = FALSE) :
only two condition levels are currently supported
Calls: aldex -> aldex.ttest
EjecuciĂłn interrumpida
Traceback (most recent call last):
File â/home/cristian/miniconda3/envs/qiime2-2020.2/lib/python3.6/site-packages/q2_aldex2/_method.pyâ, line 59, in aldex2
run_commands([cmd])
File â/home/cristian/miniconda3/envs/qiime2-2020.2/lib/python3.6/site-packages/q2_aldex2/_method.pyâ, line 21, in run_commands
subprocess.run(cmd, check=True)
File â/home/cristian/miniconda3/envs/qiime2-2020.2/lib/python3.6/subprocess.pyâ, line 418, in run
output=stdout, stderr=stderr)
subprocess.CalledProcessError: Command â[ârun_aldex2.Râ, â/tmp/tmpiwex_o1o/input.tsv.biomâ, â/tmp/tmpiwex_o1o/input.map.txtâ, âsampleâ, â128â, âtâ, âallâ, â/tmp/tmpiwex_o1o/output.summary.txtâ]â returned non-zero exit status 1.
During handling of the above exception, another exception occurred:
Traceback (most recent call last):
File â/home/cristian/miniconda3/envs/qiime2-2020.2/lib/python3.6/site-packages/q2cli/commands.pyâ, line 328, in call
results = action(**arguments)
File â</home/cristian/miniconda3/envs/qiime2-2020.2/lib/python3.6/site-packages/decorator.py:decorator-gen-477>â, line 2, in aldex2
File â/home/cristian/miniconda3/envs/qiime2-2020.2/lib/python3.6/site-packages/qiime2/sdk/action.pyâ, line 245, in bound_callable
output_types, provenance)
File â/home/cristian/miniconda3/envs/qiime2-2020.2/lib/python3.6/site-packages/qiime2/sdk/action.pyâ, line 390, in callable_executor
output_views = self._callable(**view_args)
File â/home/cristian/miniconda3/envs/qiime2-2020.2/lib/python3.6/site-packages/q2_aldex2/_method.pyâ, line 63, in aldex2
" and stderr to learn more." % e.returncode)
Exception: An error was encountered while running ALDEx2 in R (return code 1), please inspect stdout and stderr to learn more.
Could you give some advice to solve it??
Thank you to all!!
Cristian