Problem When Classifying ITS Sequences (k__Fungi;__;__;__;__;__;__)

Hi,

I'm pretty new to Qiime2 and have been using it for ITS sequence classification. I have an issue as when I am classifying my sequences i keep getting large amounts of barplots returning:
"k__Fungi;;;;;;"
This is 99% in some cases. I presume I have done something wrong and I have no idea what it is.
Appreciate any help I can get.

Thanks,
F

Hi @q2asd ,
Welcome to the forum!

This is a common issue with fungal sequences — it could indicate one of a few different possible problems either with the query sequences or the classifier (e.g., wrong input sequences, wrong orientation). I recommend checking out the topics here as a starting point for troubleshooting different possible issues:
https://forum.qiime2.org/search?q=fungi%20classification%20kingdom%20level

Please let us know what you find. Good luck!

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