# Problem in Alpha and beta diversity analysis core metrics phylogeny

**URL:** https://forum.qiime2.org/t/problem-in-alpha-and-beta-diversity-analysis-core-metrics-phylogeny/32986
**Category:** User Support
**Created:** [April 12, 2025, 5:35pm UTC](https://forum.qiime2.org/t/problem-in-alpha-and-beta-diversity-analysis-core-metrics-phylogeny/32986 "2025-04-12T17:35:09Z")
**Posts on this page:** 3
**Page:** 1

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### Author: ![Subhasish\_Sarkar](https://forum.qiime2.org/user_avatar/forum.qiime2.org/subhasish_sarkar/32/20825_2.png) [@Subhasish\_Sarkar](https://forum.qiime2.org/u/Subhasish_Sarkar)
#### Post date: [April 12, 2025, 5:35pm UTC](https://forum.qiime2.org/t/problem-in-alpha-and-beta-diversity-analysis-core-metrics-phylogeny/32986/1 "2025-04-12T17:35:09Z")

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Hello, I am new to qiime2 and i am trying to learn. I have one sample and I am stuck on the stage Alpha and beta diversity analysis, core metrics phylogeny. I ran the command  
qiime diversity core-metrics-phylogenetic   
--i-phylogeny rooted-tree.qza   
--i-table table.qza   
--p-sampling-depth 46132   
--m-metadata-file sample-metadata.tsv   
--output-dir core-metrics-results

and got the result: Plugin error from diversity:  
Ordinations with less than two dimensions are not supported.  
Debug info has been saved to /tmp/qiime2-q2cli-err-fh14mfp0.log

 ![Screenshot_1](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/8/2/828f82a1b386de93f57d774d4450cd6c56833d94.jpeg)  
How to proceed further? and if I can't do ordination because of one sample, then what to do next?

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### Author: ![timanix](https://forum.qiime2.org/user_avatar/forum.qiime2.org/timanix/32/17879_2.png) [@timanix](https://forum.qiime2.org/u/timanix)
#### Post date: [April 12, 2025, 6:56pm UTC](https://forum.qiime2.org/t/problem-in-alpha-and-beta-diversity-analysis-core-metrics-phylogeny/32986/2 "2025-04-12T18:56:51Z")

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Hello and welcome to the forum!

> [@Subhasish\_Sarkar](#):
>
> I have one sample and I am stuck on the stage Alpha and beta diversity analysis, core metrics phylogeny.

One can not calculate beta diversity (dissimilarity/similarity between samples). It is why you are getting this error.  
If you still need alpha diversity metrics, check out available plugins. There is a plugin that will calculate the metric of your choice (but I am not sure if it will work for one sample - I have never tried it).

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [May 14, 2025, 12:57am UTC](https://forum.qiime2.org/t/problem-in-alpha-and-beta-diversity-analysis-core-metrics-phylogeny/32986/3 "2025-05-14T00:57:40Z")

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