I'm trying to import paired end fastq using manifest phred 33, but I keep getting the following problem:
There was a problem importing testmanifest.tsv:
testmanifest.tsv is not a(n) PairedEndFastqManifestPhred33V2 file:
Filepath on line 1 and column "forward-absolute-filepath" could not be found (C:\Users\micro\OneDrive\Área de Trabalho\AC07_H_L001_R1_001.fastq) for sample "AC07_H".
I see there are other posts about this kind of problem, which usually can be fixed by correcting typos, but I cannot find any typos. Could anyone help me, please?
form the terminal where you running the qiime2 command, could you open the missing R1 file (as an example)?
What system are you using to run qiime2? If it is a virtual machine on your Win computer, did you allow the virual machine to have acces to the sequence folder?
In fact, now that I look better, you have your files on OneDrive, which is a Windows cloud space. Would you e able to download the files (at least few samples) on your local machine to see if it makes any difference?
Also, as unrequested suggestion, using spaces in file/folders names it may couse you trouble especially using unix command or scripts, I would remove them from your file folder name!
Sorry again, could you tell me how I would do it, please?
This is something very annoying I've been trying to figure out for a while now. I have already disconected OneDrive from my pc. I have tried to uninstall it, but apparently it can't be done.
The thing is, I have already downloaded the files on my local machine, but the desktop folder is entirely inside the OneDrive folder and I don't know how to undo this. I don't know if it means my files are in the cloud even though OneDrive is disconected.
Would you be able to help me on those matters, please?
Thank you for the suggestion! The paths you see on that print are the second attempt to make the import work - on the first attempt I had paths with no spaces and the same error