# Problem creating QIIME 2 file extension using the MiSeq Dataset without Barcode

**URL:** https://forum.qiime2.org/t/problem-creating-qiime-2-file-extension-using-the-miseq-dataset-without-barcode/3286
**Category:** User Support
**Tags:** import
**Created:** [March 2, 2018, 3:37pm UTC](https://forum.qiime2.org/t/problem-creating-qiime-2-file-extension-using-the-miseq-dataset-without-barcode/3286 "2018-03-02T15:37:32Z")
**Posts on this page:** 12
**Page:** 1

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### Author: ![dheeman00](https://forum.qiime2.org/user_avatar/forum.qiime2.org/dheeman00/32/1317_2.png) [@dheeman00](https://forum.qiime2.org/u/dheeman00)
#### Post date: [March 2, 2018, 3:37pm UTC](https://forum.qiime2.org/t/problem-creating-qiime-2-file-extension-using-the-miseq-dataset-without-barcode/3286/1 "2018-03-02T15:37:32Z")

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Hi All

I got currently working on a MiSeq Dataset 16S which does not have a separate barcode file in the folder. I know that the barcode information are already in the sequence file as the name of each files are in the following format:

A1\_S1\_L001\_R1\_001.fastq.gz  
A1\_S1\_L001\_R2\_001.fastq.gz

There are 145 of such files. I have actually browsed through some of the previous suggestions as mentioned in the website ([Problems with fastq files paired end without barcode file](https://forum.qiime2.org/t/problems-with-fastq-files-paired-end-without-barcode-file/960)). But still not able to find my required solution.

I am actually trying to create qza file extension using all the sequence data in the folder. It will be really helpful for me to anyone share some suggestions.

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### Author: ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [March 2, 2018, 4:51pm UTC](https://forum.qiime2.org/t/problem-creating-qiime-2-file-extension-using-the-miseq-dataset-without-barcode/3286/3 "2018-03-02T16:51:02Z")

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Hi @dheeman00,  
Importing different data types is described in the [QIIME2 tutorials page](https://docs.qiime2.org/2018.2/tutorials/) — in particular, it looks like you have Casava 1.8 paired-end demultiplexed fastq files, which you can import as described [here](https://docs.qiime2.org/2018.2/tutorials/importing/#casava-1-8-paired-end-demultiplexed-fastq).

You would place all of these files (and nothing else) in a single folder, then import that entire folder as a QZA as described in that tutorial.

Good luck!

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### Author: ![dheeman00](https://forum.qiime2.org/user_avatar/forum.qiime2.org/dheeman00/32/1317_2.png) [@dheeman00](https://forum.qiime2.org/u/dheeman00)
#### Post date: [March 5, 2018, 2:23pm UTC](https://forum.qiime2.org/t/problem-creating-qiime-2-file-extension-using-the-miseq-dataset-without-barcode/3286/5 "2018-03-05T14:23:29Z")

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Hi Nicholas

Thank you for the suggestion. Just have a query can I keep any other file formats like .txt or .csv files along with fastq.gz files in that same folder. Those files are just summary of the barcode info.

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### Author: ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [March 5, 2018, 2:33pm UTC](https://forum.qiime2.org/t/problem-creating-qiime-2-file-extension-using-the-miseq-dataset-without-barcode/3286/9 "2018-03-05T14:33:00Z")

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### Author: ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [March 5, 2018, 3:23pm UTC](https://forum.qiime2.org/t/problem-creating-qiime-2-file-extension-using-the-miseq-dataset-without-barcode/3286/10 "2018-03-05T15:23:27Z")

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> [@dheeman00](#):
>
> Thank you for the suggestion. Just have a query can I keep any other file formats like .txt or .csv files along with fastq.gz files in that same folder. Those files are just summary of the barcode info.

I think any non-fastq file may cause an error while importing, because QIIME2 does not expect these file types with this command. But I am not certain — text files may be okay. If in doubt, give it a try and see what happens!

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### Author: ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [March 5, 2018, 3:23pm UTC](https://forum.qiime2.org/t/problem-creating-qiime-2-file-extension-using-the-miseq-dataset-without-barcode/3286/11 "2018-03-05T15:23:28Z")

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### Author: ![dheeman00](https://forum.qiime2.org/user_avatar/forum.qiime2.org/dheeman00/32/1317_2.png) [@dheeman00](https://forum.qiime2.org/u/dheeman00)
#### Post date: [March 6, 2018, 2:50pm UTC](https://forum.qiime2.org/t/problem-creating-qiime-2-file-extension-using-the-miseq-dataset-without-barcode/3286/12 "2018-03-06T14:50:10Z")

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Hi Nicholas

I was able to generate the qza files using the dataset. Thank you for your help. But can you share some suggestions how I can view the contents inside the qza files.

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### Author: ![ebolyen](https://forum.qiime2.org/user_avatar/forum.qiime2.org/ebolyen/32/11_2.png) [@ebolyen](https://forum.qiime2.org/u/ebolyen)
#### Post date: [March 6, 2018, 3:31pm UTC](https://forum.qiime2.org/t/problem-creating-qiime-2-file-extension-using-the-miseq-dataset-without-barcode/3286/13 "2018-03-06T15:31:43Z")

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### Author: ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [March 6, 2018, 3:50pm UTC](https://forum.qiime2.org/t/problem-creating-qiime-2-file-extension-using-the-miseq-dataset-without-barcode/3286/14 "2018-03-06T15:50:26Z")

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Hi @dheeman00,  
A QZA is really just a zipped archive containing specific files that QIIME2 recognizes. So you can simply unzip a QZA to look inside, but the proper way to examine the contents is to use:

```auto
qiime tools export

```

See [this tutorial](https://docs.qiime2.org/2018.2/tutorials/exporting/) for more details.

There are specific methods for viewing and summarizing different QZA contents, depending on the file type. [This tutorial](https://docs.qiime2.org/2018.2/tutorials/moving-pictures/) covers most of the basic viewers — any method called `tabulate` or `summarize` is going to be a method for viewing the contents of a QZA in a more direct manner than unzipping/exporting the file.

It sounds like you are just getting started in QIIME2. You should check out our [getting started guide](https://docs.qiime2.org/2018.2/getting-started/) and try out some of the tutorials to get a hang of how QIIME2 works, particularly importing/exporting and working with QZA files.

I hope that helps!

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### Author: ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [March 6, 2018, 3:50pm UTC](https://forum.qiime2.org/t/problem-creating-qiime-2-file-extension-using-the-miseq-dataset-without-barcode/3286/15 "2018-03-06T15:50:27Z")

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<div class="post-metadata">

### Author: ![dheeman00](https://forum.qiime2.org/user_avatar/forum.qiime2.org/dheeman00/32/1317_2.png) [@dheeman00](https://forum.qiime2.org/u/dheeman00)
#### Post date: [March 7, 2018, 3:04pm UTC](https://forum.qiime2.org/t/problem-creating-qiime-2-file-extension-using-the-miseq-dataset-without-barcode/3286/16 "2018-03-07T15:04:04Z")

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> [@Nicholas\_Bokulich](#):
>
> s like you are just getting started in QIIME2. You should che

Thank you for your help. I did go through some of the materials and they are helpful.

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [April 7, 2018, 9:04pm UTC](https://forum.qiime2.org/t/problem-creating-qiime-2-file-extension-using-the-miseq-dataset-without-barcode/3286/17 "2018-04-07T21:04:15Z")

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