problem after demultiplexing code

[sadaffaheem (Sadaf Shabbir)]

I am stuck at a point
I run this command
time qiime deblur denoise-16S
--i-demultiplexed-seqs demux-filtered.qza
--p-trim-length 200
--o-representative-sequences rep-seqs-deblur.qza
--o-table table-deblur.qza
--p-sample-stats
--o-stats deblur-stats.qza

and I get table-deblur.qza, rep-seqs-deblur.qza and deblur-stats.qza

however, when I run the next command
time qiime feature-table tabulate-seqs
--i-data rep-seqs-deblur.qza
--o-visualization rep-seqs.qzv

I get this message
Plugin error from feature-table:

No values provided.

Debug info has been saved to /tmp/qiime2-q2cli-err-0v7d9ssk.log,

what should I do to solve this issue now? I am also attaching a piture of it

[timanix (Timur Yergaliyev)]

Hello and welcome back to the forum!
What is the length of your reads after demux and primer removal? If they are shorter than

--p-trim-length 200

then it is possible that deblur discarded all of them. In that case, you need to set a lower value for trimming or disable it to keep the reads.

[sadaffaheem (Sadaf Shabbir)]

@timanix

then it is possible that deblur discarded all of them. In that case, you need to set a lower value for trimming or disable it to keep the reads.

I tried, 80,90, 100, even 0. same error again and again , I have selected length from the visualization of demux.qzv file, visualized at the start, I am sending you and image of that as well, please guide me, I will really be thankful

[timanix (Timur Yergaliyev)]

Hi @sadaffaheem,
That's looks strange for me. It should work as it is and you tried different trimming values.
Could you share with us the content of the log file from the error (/tmp/qiime2-q2cli-err-0v7d9ssk.log), or rerun it in the verbose mode?

[sadaffaheem (Sadaf Shabbir)]

@timanix

(/tmp/qiime2-q2cli-err-0v7d9ssk.log)

I will check but I really do not know where this file goes, I am sorry, but I will try to sort out soon

[timanix (Timur Yergaliyev)]

On Ubuntu, you can open it by runing:

gedit /tmp/qiime2-q2cli-err-0v7d9ssk.log

Or replace gedit with any installed text editor.

Another way is to add --verbose flag to the command, so everything will be printed in the terminal.

In addition, could you also share deblur outputs?