# Prepping Demultiplexed Illumina Data with barcodes already removed

**URL:** https://forum.qiime2.org/t/prepping-demultiplexed-illumina-data-with-barcodes-already-removed/4237
**Category:** User Support
**Created:** [May 22, 2018, 12:54pm UTC](https://forum.qiime2.org/t/prepping-demultiplexed-illumina-data-with-barcodes-already-removed/4237 "2018-05-22T12:54:10Z")
**Posts on this page:** 5
**Page:** 1

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### Author: ![mmlopezu](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/m/ccd318/32.png) [@mmlopezu](https://forum.qiime2.org/u/mmlopezu)
#### Post date: [May 22, 2018, 12:54pm UTC](https://forum.qiime2.org/t/prepping-demultiplexed-illumina-data-with-barcodes-already-removed/4237/1 "2018-05-22T12:54:11Z")

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Hi,

I am trying to analyze Illumina data in qiime, and I was hoping I could have some advice on how to get started. The data I received is paired-end, it has already been demultiplexed and it does not contain barcodes. I am having a hard time getting the data setup to run qiime because I do not have a barcodes file to get started. I am happy to send a couple of sample files if that would be useful.

Thanks in advance for any advice.

Best,  
Margarita

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### Author: ![thermokarst](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/t/8e7dd6/32.png) [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [May 22, 2018, 1:07pm UTC](https://forum.qiime2.org/t/prepping-demultiplexed-illumina-data-with-barcodes-already-removed/4237/2 "2018-05-22T13:07:41Z")

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### Author: ![Mehrbod\_Estaki](https://forum.qiime2.org/user_avatar/forum.qiime2.org/mehrbod_estaki/32/4001_2.png) [@Mehrbod\_Estaki](https://forum.qiime2.org/u/Mehrbod_Estaki)
#### Post date: [May 22, 2018, 5:38pm UTC](https://forum.qiime2.org/t/prepping-demultiplexed-illumina-data-with-barcodes-already-removed/4237/3 "2018-05-22T17:38:45Z")

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Hi @mmlopezu,

Sounds like your files are already demultiplexed, meaning no barcode is necessary, so you can use a manifest to import those files into qiime2. Try the [manifest import tutorial here](https://docs.qiime2.org/2018.4/tutorials/importing/#fastq-manifest-formats) and let us know if you run into any problems.  
Good luck!

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### Author: ![thermokarst](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/t/8e7dd6/32.png) [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [May 22, 2018, 5:43pm UTC](https://forum.qiime2.org/t/prepping-demultiplexed-illumina-data-with-barcodes-already-removed/4237/4 "2018-05-22T17:43:27Z")

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [June 22, 2018, 11:57pm UTC](https://forum.qiime2.org/t/prepping-demultiplexed-illumina-data-with-barcodes-already-removed/4237/5 "2018-06-22T23:57:03Z")

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