Hi, Can anyone give me an example to prepare metadata for pair-end sequencing data? The tutorial is for single-end sequencing data. “Moving Pictures” tutorial — QIIME 2 2020.2.0 documentation Thanks!

[image] Huiyun_Wu: I only have the forward and reserve fastq.gz file available for my project. I have the sample ID and barcode sequence information. Is there a tutorial illustrating how to prepare the barcode fastq.gz file It sounds like you might have multiplexed-paired end reads with the b…

Welcome to the forum, @Huiyun_Wu ! You can find examples of working with paired-end sequence data in a few different places. Most importantly, keep in mind that you can get help directly from :qiime2: on the command line: ➜ qiime tools import --help Usage: qiime tools import [OPTIONS] Import da…

Thanks Andrew! I will try it out!

Something to keep in mind, @Huiyun_Wu is that there aren't really any requirements in QIIME 2 regarding sample metadata (all of the old QIIME 1 requirements about linker-primer-sequence et al all gone): https://docs.qiime2.org/2020.2/tutorials/metadata/ Most metadata-needs will be driven by your s…

Hi Andrew, I was following the "Atacama soil" tutorial “Atacama soil microbiome” tutorial — QIIME 2 2020.2.0 documentation . The barcode fastq.gz file is already prepared and easy to download. However, I only have the forward and reserve fastq.gz file available for my project. I have the sample ID a…

QIIME 2 Forum

Prepare Metadata for pair-end sequencing data

Technical Support

show post in topic

Home
Categories
Guidelines
Terms of Service
Privacy Policy

Powered by Discourse, best viewed with JavaScript enabled