I’m following the steps of the FMT Transplant tutorial, but using a subset of data from one of my own studies. At the feature-table summarize step, I am getting the following error [truncated for brevity]
Plugin error from feature-table:
"None of [Index([‘S22-indexN719-B-S508-B-GCGTAGTA-CTAAGCCT-F3’,\n
dtype=‘object’)] are in the [index]"
These are the beginnings of the fastq file names from a MiSeq run, which I had imported to qiime2 using the steps for Casava 1.8 paired end demultiplexed fastq laid out in the “Importing Data” tutorial. The only thing that had happened to these files is they were renamed with a sample ID at the front of the file name (i.e. S01, S02 etc etc). The sample ID’s in the mapping file are also S01, S02 etc. The mapping file validates with Keemi with no problems.
In the full file name, there is also a sample ID towards the end of the name, as illustrated below for S01
The original files from the sequencing facility all started with a common lane designator, as below
Could this be a problem due to the renaming of the read files? Did I actually use the wrong import methodology?
I have analyzed these data from these files using qiime 1 with no problems.
Thanks in advance for any suggestions or advice.