Dear Team,
I just installed the latest QIIME2 version using ubuntu 20.04 LTS in WSL2. I was trying the "Moving pictures" tutorial to check if the installation working properly. At the Alpha and beta diversity analysis stage I am running in to the following error.
"Plugin error from diversity:
[('/tmp/qiime2-archive-37dumjet/81242a89-b421-4fa8-9858-26df8ce1b39f/data/feature-table.biom', '/tmp/qiime2-archive-zeujrk6z/d25377eb-8730-4d57-bf86-452a2ef5cbf3/data/feature-table.biom', "[Errno 13] Permission denied: '/tmp/qiime2-archive-37dumjet/81242a89-b421-4fa8-9858-26df8ce1b39f/data/feature-table.biom' -> '/tmp/qiime2-archive-zeujrk6z/d25377eb-8730-4d57-bf86-452a2ef5cbf3/data/feature-table.biom'")]
Debug info has been saved to /tmp/qiime2-q2cli-err-mxn8zm9n.log"
the log file has the following message.
"/home/dhinothb/miniconda3/envs/qiime2-2021.4/lib/python3.8/site-packages/sklearn/metrics/pairwise.py:1776: DataConversionWarning: Data was converted to boolean for metric jaccard
warnings.warn(msg, DataConversionWarning)
/home/dhinothb/miniconda3/envs/qiime2-2021.4/lib/python3.8/site-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:143: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.012488721124875216 and the largest is 3.591392193348517.
warn(
/home/dhinothb/miniconda3/envs/qiime2-2021.4/lib/python3.8/site-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:143: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.012972069562952403 and the largest is 2.4367036221169256.
warn(
/home/dhinothb/miniconda3/envs/qiime2-2021.4/lib/python3.8/site-packages/skbio/stats/ordination/_principal_coordinate_analysis.py:143: RuntimeWarning: The result contains negative eigenvalues. Please compare their magnitude with the magnitude of some of the largest positive eigenvalues. If the negative ones are smaller, it's probably safe to ignore them, but if they are large in magnitude, the results won't be useful. See the Notes section for more details. The smallest eigenvalue is -0.018973045082089985 and the largest is 3.159951574009762.
warn(
Traceback (most recent call last):
File "/home/dhinothb/miniconda3/envs/qiime2-2021.4/lib/python3.8/site-packages/q2cli/commands.py", line 329, in call
results = action(**arguments)
File "", line 2, in core_metrics_phylogenetic
File "/home/dhinothb/miniconda3/envs/qiime2-2021.4/lib/python3.8/site-packages/qiime2/sdk/action.py", line 244, in bound_callable
outputs = self.callable_executor(scope, callable_args,
File "/home/dhinothb/miniconda3/envs/qiime2-2021.4/lib/python3.8/site-packages/qiime2/sdk/action.py", line 512, in callable_executor
aliased_result = output._alias(prov)
File "/home/dhinothb/miniconda3/envs/qiime2-2021.4/lib/python3.8/site-packages/qiime2/sdk/result.py", line 162, in _alias
alias._archiver = archive.Archiver.from_data(
File "/home/dhinothb/miniconda3/envs/qiime2-2021.4/lib/python3.8/site-packages/qiime2/core/archive/archiver.py", line 316, in from_data
Format.write(rec, type, format, data_initializer, provenance_capture)
File "/home/dhinothb/miniconda3/envs/qiime2-2021.4/lib/python3.8/site-packages/qiime2/core/archive/format/v5.py", line 20, in write
super().write(archive_record, type, format, data_initializer,
File "/home/dhinothb/miniconda3/envs/qiime2-2021.4/lib/python3.8/site-packages/qiime2/core/archive/format/v1.py", line 18, in write
super().write(archive_record, type, format, data_initializer,
File "/home/dhinothb/miniconda3/envs/qiime2-2021.4/lib/python3.8/site-packages/qiime2/core/archive/format/v0.py", line 62, in write
data_initializer(data_dir)
File "/home/dhinothb/miniconda3/envs/qiime2-2021.4/lib/python3.8/site-packages/qiime2/sdk/result.py", line 157, in clone_original
shutil.copytree(str(self._archiver.data_dir), str(into),
File "/home/dhinothb/miniconda3/envs/qiime2-2021.4/lib/python3.8/shutil.py", line 557, in copytree
return _copytree(entries=entries, src=src, dst=dst, symlinks=symlinks,
File "/home/dhinothb/miniconda3/envs/qiime2-2021.4/lib/python3.8/shutil.py", line 513, in _copytree
raise Error(errors)
shutil.Error: [('/tmp/qiime2-archive-4s9no9s9/bb891194-6c2c-42f9-8c81-924037306b5c/data/feature-table.biom', '/tmp/qiime2-archive-gjq55yzf/aa1376ec-9b13-42ba-85b0-e321ce8deca5/data/feature-table.biom', "[Errno 13] Permission denied: '/tmp/qiime2-archive-4s9no9s9/bb891194-6c2c-42f9-8c81-924037306b5c/data/feature-table.biom' -> '/tmp/qiime2-archive-gjq55yzf/aa1376ec-9b13-42ba-85b0-e321ce8deca5/data/feature-table.biom'")]"
PLease help me to rectify this.
Thanks