# Plugin error from diversity (on rarefaction)
**URL:** https://forum.qiime2.org/t/plugin-error-from-diversity-on-rarefaction/2609
**Category:** User Support
**Tags:** alpha-rarefaction, metadata
**Created:** [January 17, 2018, 4:38pm UTC](https://forum.qiime2.org/t/plugin-error-from-diversity-on-rarefaction/2609 "2018-01-17T16:38:40Z")
**Posts on this page:** 7
**Page:** 1
### Author:  [@Restuadi](https://forum.qiime2.org/u/Restuadi)
#### Post date: [January 17, 2018, 4:38pm UTC](https://forum.qiime2.org/t/plugin-error-from-diversity-on-rarefaction/2609/1 "2018-01-17T16:38:40Z")
Hello,
I guess there were some discussion about my error here, but they are closed, and my situation is somewhat different, I got errors on rarefaction.
Ok this is my command and what I got :
(qiime2-2017.11) [r.restuadi@delta017 fastq\_2]$ qiime diversity alpha-rarefaction \
> --i-table table\_sh\_10thr\_2.qza
> --i-phylogeny rooted-tree.qza
> --p-max-depth 4000
> --m-metadata-file mapping\_16Jan2018.txt
> --o-visualization alpha-rarefaction\_4k.qzv
Plugin error from diversity:
Missing samples in metadata: {'R4286', 'R2406'}
Debug info has been saved to /scratch/tmp/pbs.1509491.delta/qiime2-q2cli-err-27usq86l.log
I've checked that my mapping is containing those 2 samples, and they are in table\_sh\_10thr\_2.qza too.
Could you help me to figure out, what's wrong here?
Thank you very much,
Restu
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### Author:  [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [January 17, 2018, 5:33pm UTC](https://forum.qiime2.org/t/plugin-error-from-diversity-on-rarefaction/2609/3 "2018-01-17T17:33:57Z")
Hi @Restuadi!
Are those sample ids listed in exactly the same way in the metadata file and your table? ID matching is exact, so if you have hyphens versus underscores, or upper/lowercase letters, those will be read as different IDs (please note, the metadata behavior described here might not be applicable in future releases of QIIME 2 - we are currently overhauling the metadata handling in the framework).
Some ways you can make sure your IDs match:
- Run [`feature-table summarize`](https://docs.qiime2.org/2017.12/plugins/available/feature-table/summarize/) on your feature table, without your metadata, you will have a list of sample IDs in there.
- Run [`feature-table summarize`](https://docs.qiime2.org/2017.12/plugins/available/feature-table/summarize/) on your feature table, with your metadata. Compare the sample IDs in this step with the sample IDs in the previous step.
- Run [`metadata tabulate`](https://docs.qiime2.org/2017.12/plugins/available/metadata/tabulate/) on your metadata.
If you are still stuck after investigating those three files, can you please provide us with the three visualizations above? Thanks! 🦖
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### Author:  [@Restuadi](https://forum.qiime2.org/u/Restuadi)
#### Post date: [January 18, 2018, 4:01am UTC](https://forum.qiime2.org/t/plugin-error-from-diversity-on-rarefaction/2609/5 "2018-01-18T04:01:11Z")
Hi,
Yes, I guess, I'm still pretty stuck, since the number seems to match.
I cannot find any attachment functionality in this forum, so for the time being, I put the visualization here:
[https://drive.google.com/open?id=1pVzHVuFq0kTzTavdIPQbbOdXJaIEef-w](https://drive.google.com/open?id=1pVzHVuFq0kTzTavdIPQbbOdXJaIEef-w)
Please let me know if you need anything else,
thank you very much,
Restu
---
### Author:  [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [January 18, 2018, 4:05am UTC](https://forum.qiime2.org/t/plugin-error-from-diversity-on-rarefaction/2609/6 "2018-01-18T04:05:02Z")
Hi @Restuadi! Thanks for the files - that was super helpful!
You have extra spaces (`' '`) after the listed sample IDs in your metadata file (`'R4286 '` instead of `'R4286'` & `'R2406 '` instead of `'R2406'`). If you delete those spaces in your metadata file, you should be good to go.
We are currently in the middle of overhauling metadata handling in QIIME 2 --- once those changes land, one important change is that leading and trailing whitespace will not be significant --- it will be stripped off, so this wouldn't have even been an issue for you. We will let you know when this is made available in a future release - stay tuned!
Thanks 🦖
PS - you can attach files by clicking this icon in the edit menu bar:
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### Author:  [@Restuadi](https://forum.qiime2.org/u/Restuadi)
#### Post date: [January 18, 2018, 12:42pm UTC](https://forum.qiime2.org/t/plugin-error-from-diversity-on-rarefaction/2609/7 "2018-01-18T12:42:36Z")
YES...! It Works.
Awesome, Thank you very much.
Restu
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### Author:  [@jairideout](https://forum.qiime2.org/u/jairideout)
#### Post date: [February 16, 2018, 5:12pm UTC](https://forum.qiime2.org/t/plugin-error-from-diversity-on-rarefaction/2609/9 "2018-02-16T17:12:35Z")
In the QIIME 2 `2018.2` release, Metadata ignores any leading or trailing whitespace characters in cells, avoiding the issue reported in this topic.
There are a number of other changes to QIIME 2 Metadata in the `2018.2` release. See [this forum announcement](https://forum.qiime2.org/t/summary-of-changes-to-metadata-in-qiime-2-2018-2-release/2884) for details on what changed, as well as the updated [Metadata tutorial](https://docs.qiime2.org/2018.2/tutorials/metadata/). 🌞
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### Author:  [@system](https://forum.qiime2.org/u/system)
#### Post date: [March 19, 2018, 11:12pm UTC](https://forum.qiime2.org/t/plugin-error-from-diversity-on-rarefaction/2609/11 "2018-03-19T23:12:44Z")
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