I am a new user of Qiime2, and this is my first time to use and Qiime related bio-informatics. I tried to practice “Moving Pictures” tutorial in Qiime2. I installed Qiime2 using Virtualbox.
Plugin error from demux:
/tmp/qiime2-archive-qg65j891/426c5b49-3232-414a-8a0b-0192ac6e1a79/data/barcodes.fastq.gz is not a file.
Debug info has been saved to /tmp/qiime2-q2cli-err-0pxdpd0g.log
Could any one who know this can deliver a solution or pointing out the reason for the error.
I tried many time of testing, such as reinstall Qiime2 using VirtualBox, and change the location of shared folder, but none of them worked. Please help…
Should close this request. My colleague helped to fix this issue. After reset the working directory: qiime2-2019.1) qiime2@qiime2core2019-1:/media/sf_Desktop/qiime2-moving-pictures-tutorial$
Then following Moving Pictures, it worked.
I guess because I chose Desktop as shared folder, but not set a file for working.
Hmm, I am not so sure that was the problem. Take a look at the ls results above: your filenames had an extra .gz extension at the end. I am not sure why they were able to imported in the first place, but I suspect this had something to do with it.
Glad to hear you are sorted out now. Keep on QIIMEing!