# Plugin error from demux:Value must be a nonnegative integer or None

**URL:** https://forum.qiime2.org/t/plugin-error-from-demux-value-must-be-a-nonnegative-integer-or-none/31193
**Category:** User Support
**Tags:** demux
**Created:** [August 20, 2024, 1:38pm UTC](https://forum.qiime2.org/t/plugin-error-from-demux-value-must-be-a-nonnegative-integer-or-none/31193 "2024-08-20T13:38:23Z")
**Posts on this page:** 3
**Page:** 1

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### Author: ![bbiamasheep123](https://forum.qiime2.org/letter_avatar_proxy/v4/letter/b/aeb1de/32.png) [@bbiamasheep123](https://forum.qiime2.org/u/bbiamasheep123)
#### Post date: [August 20, 2024, 1:38pm UTC](https://forum.qiime2.org/t/plugin-error-from-demux-value-must-be-a-nonnegative-integer-or-none/31193/1 "2024-08-20T13:38:23Z")

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Hi all

1.time qiime feature-table summarize --i-table table.qza --m-sample-metadata-file metadata.txt --o-visualization table.qzv  
2.time qiime feature-table tabulate-seqs --i-data repseqs.qza --o-visualization repseqs.qzv

This prompts me when I successfully run the first line of code"time qiime feature-table summarize --i-table table.qza --m-sample-metadata-file metadata.txt --o-visualization table.qzv"

```auto
real 13m36.151s
user 13m34.0385
SVS 0m2.0945

```

Then I ran the second line of code,the following error message appears

```auto
Plugin error from demux:
Value must be a nonnegative integer or None
Debug info has been saved to /tmp/qiime2-q2cli-err-6lsl970n.log

```

Where is the problem? Is there a problem with the parameter Settings in demux? Or is there a problem with the raw data? How can I solve this problem?Hope you can help me.❤

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<div class="post-metadata">

### Author: ![colinvwood](https://forum.qiime2.org/user_avatar/forum.qiime2.org/colinvwood/32/17303_2.png) [@colinvwood](https://forum.qiime2.org/u/colinvwood)
#### Post date: [August 20, 2024, 2:25pm UTC](https://forum.qiime2.org/t/plugin-error-from-demux-value-must-be-a-nonnegative-integer-or-none/31193/2 "2024-08-20T14:25:18Z")

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Hello @bbiamasheep123,

Can you post the full command you ran, run it with the `--verbose` flag and then post the full error here?

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [September 20, 2024, 8:25pm UTC](https://forum.qiime2.org/t/plugin-error-from-demux-value-must-be-a-nonnegative-integer-or-none/31193/3 "2024-09-20T20:25:59Z")

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