Plugin error from demux: 'NoneType' object has no attribute 'strip'

Hello I imported my manifest file and got output as demux paired end. zip file

I ran the following command
qiime demux summarize --i-data /home/temp/Desktop/paired-end-demux.qza --o-visualization /home/temp/Desktop/paired-end-demux.qzv

Got following error
Plugin error from demux:

'NoneType' object has no attribute 'strip'

I did try one the solution mentioned here on the forum by user thermokarst. However it did not work for me.

Kindly help

Hi @komal,

Thanks for reaching out! Do you mind sharing your demux file? I suspect it may be empty, which is why you're receiving that NoneType object error message.

Hello, Thank you for your relpy
Kindly find attached snap of demux paired end file (extracted from zip)


Hi @komal,

Do you mind sharing the actual file so I can try to reproduce the error on my end? If you'd prefer to send via DM vs over this thread, that's perfectly fine.

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