Hello, I’m a new user of Qiime2. Please I’d like some help. I was trying to analyze some NGS data, but when running demux plugin:
$ qiime demux emp-paired --m-barcodes-file metafood.tsv --m-barcodes-column BarcodeSequence --p-no-golay-error-correction --i-seqs ncbi-pairend-seqs.qza --o-per-sample-sequences demux-ncbi.qza --o-error-correction-details demux-details-ncbi.qza
it stops after 30 minutes, and shows the error:
Plugin error from demux:
could not write to ‘/tmp/qiime2-archive-f6vjw_9f/98154bea-d272-4029-bc9a-4cc772f456a7/data/ggEDEJ046_159_L001_R2_001.fastq.gz’: No space left on device
I did a native installation on my Linux. I have a core i7 laptop with 16 gb ram. My error log file:
Traceback (most recent call last):
File “/home/jesus/anaconda3/envs/qiime2-2020.6/lib/python3.6/distutils/file_util.py”, line 57, in _copy_file_contents
fdst.write(buf)
OSError: [Errno 28] No space left on device
During handling of the above exception, another exception occurred:
Traceback (most recent call last):
File “/home/jesus/anaconda3/envs/qiime2-2020.6/lib/python3.6/site-packages/q2cli/commands.py”, line 329, in call
results = action(**arguments)
File “”, line 2, in emp_paired
File “/home/jesus/anaconda3/envs/qiime2-2020.6/lib/python3.6/site-packages/qiime2/sdk/action.py”, line 245, in bound_callable
output_types, provenance)
File “/home/jesus/anaconda3/envs/qiime2-2020.6/lib/python3.6/site-packages/qiime2/sdk/action.py”, line 418, in callable_executor
spec.qiime_type, output_view, spec.view_type, prov)
File “/home/jesus/anaconda3/envs/qiime2-2020.6/lib/python3.6/site-packages/qiime2/sdk/result.py”, line 273, in _from_view
provenance_capture=provenance_capture)
File “/home/jesus/anaconda3/envs/qiime2-2020.6/lib/python3.6/site-packages/qiime2/core/archive/archiver.py”, line 316, in from_data
Format.write(rec, type, format, data_initializer, provenance_capture)
File “/home/jesus/anaconda3/envs/qiime2-2020.6/lib/python3.6/site-packages/qiime2/core/archive/format/v5.py”, line 21, in write
provenance_capture)
File “/home/jesus/anaconda3/envs/qiime2-2020.6/lib/python3.6/site-packages/qiime2/core/archive/format/v1.py”, line 19, in write
provenance_capture)
File “/home/jesus/anaconda3/envs/qiime2-2020.6/lib/python3.6/site-packages/qiime2/core/archive/format/v0.py”, line 62, in write
data_initializer(data_dir)
File “/home/jesus/anaconda3/envs/qiime2-2020.6/lib/python3.6/site-packages/qiime2/core/path.py”, line 33, in _move_or_copy
return distutils.dir_util.copy_tree(str(self), str(other))
File “/home/jesus/anaconda3/envs/qiime2-2020.6/lib/python3.6/distutils/dir_util.py”, line 163, in copy_tree
dry_run=dry_run)
File “/home/jesus/anaconda3/envs/qiime2-2020.6/lib/python3.6/distutils/file_util.py”, line 151, in copy_file
_copy_file_contents(src, dst)
File “/home/jesus/anaconda3/envs/qiime2-2020.6/lib/python3.6/distutils/file_util.py”, line 60, in _copy_file_contents
“could not write to ‘%s’: %s” % (dst, e.strerror))
distutils.errors.DistutilsFileError: could not write to ‘/tmp/qiime2-archive-f6vjw_9f/98154bea-d272-4029-bc9a-4cc772f456a7/data/ggEDEJ046_159_L001_R2_001.fastq.gz’: No space left on device
Thanks in advance for your replies.