Hi guys, I seem to have run into this error and I'm not sure why. Any suggestions to fix this? I have already deleted the data and downloaded the files again and am still running into the issue.
I am running qiime2-2022.8
qiime demux summarize \
--i-data /Users/sam/LT-paired-ends.qza
--o-visualization LT-paired-ends.qzv
--verbose
error:
Traceback (most recent call last):
File "/Users/sam/miniconda3/envs/qiime2-2022.8/lib/python3.8/site-packages/q2cli/commands.py", line 339, in call
results = action(**arguments)
File "", line 2, in summarize
File "/Users/sam/miniconda3/envs/qiime2-2022.8/lib/python3.8/site-packages/qiime2/sdk/action.py", line 234, in bound_callable
outputs = self.callable_executor(scope, callable_args,
File "/Users/sam/miniconda3/envs/qiime2-2022.8/lib/python3.8/site-packages/qiime2/sdk/action.py", line 443, in callable_executor
ret_val = self._callable(output_dir=temp_dir, **view_args)
File "/Users/sam/miniconda3/envs/qiime2-2022.8/lib/python3.8/site-packages/q2_demux/_summarize/_visualizer.py", line 116, in summarize
manifest = data.manifest.view(pd.DataFrame)
File "/Users/sam/miniconda3/envs/qiime2-2022.8/lib/python3.8/site-packages/qiime2/plugin/model/directory_format.py", line 78, in view
return transformation(self.path_maker())
File "/Users/sam/miniconda3/envs/qiime2-2022.8/lib/python3.8/site-packages/qiime2/core/transform.py", line 70, in transformation
new_view = transformer(view)
File "/Users/sam/miniconda3/envs/qiime2-2022.8/lib/python3.8/site-packages/q2_types/per_sample_sequences/_transformer.py", line 212, in _21
return _manifest_to_df(ff, ff.path.parent)
File "/Users/sam/miniconda3/envs/qiime2-2022.8/lib/python3.8/site-packages/q2_types/per_sample_sequences/_util.py", line 316, in _manifest_to_df
df = manifest.pivot(index='sample-id', columns='direction',
File "/Users/sam/miniconda3/envs/qiime2-2022.8/lib/python3.8/site-packages/pandas/core/frame.py", line 7885, in pivot
return pivot(self, index=index, columns=columns, values=values)
File "/Users/sam/miniconda3/envs/qiime2-2022.8/lib/python3.8/site-packages/pandas/core/reshape/pivot.py", line 520, in pivot
return indexed.unstack(columns_listlike)
File "/Users/sam/miniconda3/envs/qiime2-2022.8/lib/python3.8/site-packages/pandas/core/series.py", line 4157, in unstack
return unstack(self, level, fill_value)
File "/Users/sam/miniconda3/envs/qiime2-2022.8/lib/python3.8/site-packages/pandas/core/reshape/reshape.py", line 491, in unstack
unstacker = _Unstacker(
File "/Users/sam/miniconda3/envs/qiime2-2022.8/lib/python3.8/site-packages/pandas/core/reshape/reshape.py", line 140, in init
self._make_selectors()
File "/Users/sam/miniconda3/envs/qiime2-2022.8/lib/python3.8/site-packages/pandas/core/reshape/reshape.py", line 192, in _make_selectors
raise ValueError("Index contains duplicate entries, cannot reshape")
ValueError: Index contains duplicate entries, cannot reshape
Plugin error from demux:
Index contains duplicate entries, cannot reshape