# Plugin error from dada2: No reads passed the filter
**URL:** https://forum.qiime2.org/t/plugin-error-from-dada2-no-reads-passed-the-filter/6744
**Category:** Technical Support
**Created:** [November 2, 2018, 2:55am UTC](https://forum.qiime2.org/t/plugin-error-from-dada2-no-reads-passed-the-filter/6744 "2018-11-02T02:55:31Z")
**Posts on this page:** 5
**Page:** 1
### Author:  [@emescioglu](https://forum.qiime2.org/u/emescioglu)
#### Post date: [November 2, 2018, 2:55am UTC](https://forum.qiime2.org/t/plugin-error-from-dada2-no-reads-passed-the-filter/6744/1 "2018-11-02T02:55:31Z")
Hi all,
I saw there an old post to which replies are no longer allowed, so I figured I would post my own. I'm trying to use dada2 on f and r reads, but I have some sequences that are too short. [Here](https://drive.google.com/drive/folders/1_yAhHN7XOGFvQyr4mUOZcxPHQelQPL5V?usp=sharing) are my demux files.
I'm wondering if there are only a few reads that are too short, and if so - could I remove them to move forward? In the previous post, it was suggested that they use only their forward reads but I was wondering if there was another option.
Some background: My sequences are in the mixed orientation format and I'm re-processing using advice provided [here](https://forum.qiime2.org/t/mixed-oriented-reads/6242/6). Previously I had used cutadapt to deal with the mixed orientation issue and dada2 worked fine after that. I assume the short sequences were tossed away in that process... So here is the error I'm getting now
**Plugin error from dada2:**
**No reads passed the filter. trunc\_len\_f (237) or trunc\_len\_r (287) may be individually longer than read lengths, or trunc\_len\_f + trunc\_len\_r may be shorter than the length of the amplicon + 20 nucleotides (the length of the overlap). Alternatively, other arguments (such as max\_ee or trunc\_q) may be preventing reads from passing the filter.**
**Debug info has been saved to /var/folders/gc/t0lly6tx217bq0p81wrgmtp80000gn/T/qiime2-q2cli-err-5xu23z7q.log**
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### Author:  [@shira](https://forum.qiime2.org/u/shira)
#### Post date: [November 2, 2018, 3:43am UTC](https://forum.qiime2.org/t/plugin-error-from-dada2-no-reads-passed-the-filter/6744/2 "2018-11-02T03:43:16Z")
What is the length of your amplicon?
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### Author:  [@emescioglu](https://forum.qiime2.org/u/emescioglu)
#### Post date: [November 2, 2018, 3:55am UTC](https://forum.qiime2.org/t/plugin-error-from-dada2-no-reads-passed-the-filter/6744/3 "2018-11-02T03:55:03Z")
They are 250 basepairs
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### Author:  [@shira](https://forum.qiime2.org/u/shira)
#### Post date: [November 2, 2018, 4:24am UTC](https://forum.qiime2.org/t/plugin-error-from-dada2-no-reads-passed-the-filter/6744/4 "2018-11-02T04:24:58Z")
Try truncating both fwd and rev at 150, that should be enough.
Good luck!
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### Author:  [@system](https://forum.qiime2.org/u/system)
#### Post date: [December 3, 2018, 10:31am UTC](https://forum.qiime2.org/t/plugin-error-from-dada2-no-reads-passed-the-filter/6744/5 "2018-12-03T10:31:28Z")
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