**Hi, **
Im currently running on a linux server: (qiime2-2019.1) bash-4.1$ qiime dada2 denoise-single --i-demultiplexed-seqs demux-single-end.qza --p-trim-left 0 --p-trunc-len 125 --o-representative-sequences rep-seqs-dada2.qza --o-table table-dada2.qza --o-denoising-stats stats-dada2.qza
and have encounted this error:
Plugin error from dada2:
An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more.
Debug info has been saved to /short/dq69/js1541/tmp/qiime2-q2cli-err-3o7a9lv2.log
When looking into the log, it reads:
Running external command line application(s). This may print messages to stdout and/or stderr.
The command(s) being run are below. These commands cannot be manually re-run as they will depend on temporary files that no longer exist.
Command: run_dada_single.R /short/dq69/js1541/tmp/qiime2-archive-rwa2cfkv/a7728b0f-f74a-4de6-94cb-7de1a01fdbe9/data /short/dq69/js1541/tmp/tmpu498hn3e/output.tsv.biom /short/dq69/js1541/tmp/tmpu498hn3e/track.tsv /short/dq69/js1541/tmp/tmpu498hn3e 125 0 2.0 2 Inf consensus 1.0 1 1000000 NULL 16
R version 3.3.0 (2016-05-03)
Loading required package: Rcpp
Error in dyn.load(file, DLLpath = DLLpath, ...) :
unable to load shared object '/short/dq69/miniconda3/envs/qiime2-2019.1/lib/R/library/rlang/libs/rlang.so':
/short/dq69/miniconda3/envs/qiime2-2019.1/lib/R/library/rlang/libs/rlang.so: undefined symbol: R_ExternalPtrAddrFn
Error: package or namespace load failed for ‘dada2’
Execution halted
Traceback (most recent call last):
File "/short/dq69/miniconda3/envs/qiime2-2019.1/lib/python3.6/site-packages/q2_dada2/_denoise.py", line 152, in _denoise_single
run_commands([cmd])
File "/short/dq69/miniconda3/envs/qiime2-2019.1/lib/python3.6/site-packages/q2_dada2/_denoise.py", line 36, in run_commands
subprocess.run(cmd, check=True)
File "/short/dq69/miniconda3/envs/qiime2-2019.1/lib/python3.6/subprocess.py", line 418, in run
output=stdout, stderr=stderr)
subprocess.CalledProcessError: Command '['run_dada_single.R', '/short/dq69/js1541/tmp/qiime2-archive-rwa2cfkv/a7728b0f-f74a-4de6-94cb-7de1a01fdbe9/data', '/short/dq69/js1541/tmp/tmpu498hn3e/output.tsv.biom', '/short/dq69/js1541/tmp/tmpu498hn3e/track.tsv', '/short/dq69/js1541/tmp/tmpu498hn3e', '125', '0', '2.0', '2', 'Inf', 'consensus', '1.0', '1', '1000000', 'NULL', '16']' returned non-zero exit status 1.
During handling of the above exception, another exception occurred:
Traceback (most recent call last):
File "/short/dq69/miniconda3/envs/qiime2-2019.1/lib/python3.6/site-packages/q2cli/commands.py", line 274, in call
results = action(**arguments)
File "</short/dq69/miniconda3/envs/qiime2-2019.1/lib/python3.6/site-packages/decorator.py:decorator-gen-440>", line 2, in denoise_single
File "/short/dq69/miniconda3/envs/qiime2-2019.1/lib/python3.6/site-packages/qiime2/sdk/action.py", line 231, in bound_callable
output_types, provenance)
File "/short/dq69/miniconda3/envs/qiime2-2019.1/lib/python3.6/site-packages/qiime2/sdk/action.py", line 365, in callable_executor
output_views = self._callable(**view_args)
File "/short/dq69/miniconda3/envs/qiime2-2019.1/lib/python3.6/site-packages/q2_dada2/_denoise.py", line 187, in denoise_single
band_size='16')
File "/short/dq69/miniconda3/envs/qiime2-2019.1/lib/python3.6/site-packages/q2_dada2/_denoise.py", line 163, in _denoise_single
" and stderr to learn more." % e.returncode)
Exception: An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more.
Does this have something to do with the version of R packages available?
I am new to Qiime + Linux servers so any assistance would be greatly appreciated 
**Thanks in advance, **
Jordan
