Hi @sayed,
Looks like you are trying to run the picrust2 stand alone pipeline rather than the q2-picrust2 wrapper. Was this your intention or did you mean to use the QIIME 2 version? If indeed you are having trouble with the stand-alone version my suggestion is to contact the developer @gmdouglas on the picrust2 github page, as you’ll have a much better chance of finding support. If you meant to use the q2-picrust2 plugin then see this tutorial instead and let us know if you have specific issues related to it.
Thanks Mehrbod,
I’m trying picrust2 stand alone pipeline. I sent to developer @gmdouglas, I wish I can get a response soon.
Thanks for your care
BR
SAYED