# picrust2 input files ( for bacteria)

**URL:** https://forum.qiime2.org/t/picrust2-input-files-for-bacteria/27968
**Category:** Other Bioinformatics Tools
**Created:** [October 16, 2023, 10:29am UTC](https://forum.qiime2.org/t/picrust2-input-files-for-bacteria/27968 "2023-10-16T10:29:05Z")
**Posts on this page:** 1
**Showing post:** 3

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### Author: ![jphagen](https://forum.qiime2.org/user_avatar/forum.qiime2.org/jphagen/32/17133_2.png) [@jphagen](https://forum.qiime2.org/u/jphagen)
#### Post date: [October 16, 2023, 5:07pm UTC](https://forum.qiime2.org/t/picrust2-input-files-for-bacteria/27968/3 "2023-10-16T17:07:11Z")

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Hi @Will_Ericksen,

1. 

> [@Will\_Ericksen](#):
>
> - Can I use the ASV file (denoised using DADA2) as input instead of OTU?

Yes, according to [the tutorial](https://github.com/picrust/picrust2/wiki/PICRUSt2-Tutorial-(v2.5.2)), you can use either the ASV file or OTU.

1. 

> [@Will\_Ericksen](#):
>
> My ASV file is in .csv format. How can I convert it to a table.biom format or I just converted to .tsv and run the script?

[This](https://forum.qiime2.org/t/csv-to-a-qiime2-readable-format/2284) forum post describes how to convert .csv to a .biom table.

1. 

> [@Will\_Ericksen](#):
>
> among the input files there is metadata.tsv but this file doesn't appear in the command lines as input

You are correct! it is not used directly in the commands. However, you may need it in your downstream analysis of the piecrust output.

1. 

> [@Will\_Ericksen](#):
>
> Can I install PICRUSt2 using this command: conda create -n picrust2 -c bioconda -c conda-forge picrust2=2.5.2 instead of mamba create -n picrust2 -c bioconda -c conda-forge picrust2=2.5.2 because I don't have Mamba on my Linux?

Yes, you should be able to use that command.

1. 

> [@Will\_Ericksen](#):
>
> Does the installation of PICRUSt2 using Conda take too much time? The environment solving process has been running for 2 hours, and the package is not yet installed.

If the installation is taking too much time you may want to install mamba to make the process more efficient.  
There is also [this option](https://library.qiime2.org/plugins/q2-picrust2/13/) available to you. Which avoids a mamba install, but will need to be run within a qiime2 environment.

I hope this information is helpful to you! Feel free to reach out again if you have more questions.

-Hannah

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