I am trying to run picrust in qiime2. When I tried to execute this command
metagenome_pipeline.py -i feature-table.biom -m marker_predicted_and_nsti.tsv.gz -f EC_predicted.tsv.gz -o EC_metagenome_out --strat_out
I am getting the following errors below. Can someone please help me resolve this error?
171 of 5569 ASVs were above the max NSTI cut-off of 2.0 and were removed.
Traceback (most recent call last):
File “/home/owner/anaconda3/envs/qiime2-2019.7/bin/metagenome_pipeline.py”, line 122, in
File “/home/owner/anaconda3/envs/qiime2-2019.7/bin/metagenome_pipeline.py”, line 104, in main
File “/home/owner/anaconda3/envs/qiime2-2019.7/lib/python3.6/site-packages/picrust2/metagenome_pipeline.py”, line 68, in run_metagenome_pipeline
File “/home/owner/anaconda3/envs/qiime2-2019.7/lib/python3.6/site-packages/picrust2/util.py”, line 358, in three_df_index_overlap_sort
ValueError: No sequence ids overlap between all three of the input files.