# PICRUSt and Qiime2
**URL:** https://forum.qiime2.org/t/picrust-and-qiime2/4957
**Category:** User Support
**Tags:** feature-classifier, closed-reference, biom, picrust
**Created:** [July 9, 2018, 7:55pm UTC](https://forum.qiime2.org/t/picrust-and-qiime2/4957 "2018-07-09T19:55:00Z")
**Posts on this page:** 6
**Page:** 1
### Author:  [@ellen](https://forum.qiime2.org/u/ellen)
#### Post date: [July 9, 2018, 7:55pm UTC](https://forum.qiime2.org/t/picrust-and-qiime2/4957/1 "2018-07-09T19:55:00Z")
Hello,
I am trying to make a .biom file suitable for use in PICRUSt; however, I can not find reference sequences (would be apart of the **--i-reference-sequences** command in the **qiime vsearch cluster-features-closed-reference** plugin). PICRUSt is trained on gg\_13\_5 and I can find a file called **gg\_13\_5\_otus.tar.qz** but cannot find or figure how to convert that file to **gg\_13\_5\_otus.qza**. If could you give me some direction, that would be very helpful.
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### Author:  [@ellen](https://forum.qiime2.org/u/ellen)
#### Post date: [July 9, 2018, 8:05pm UTC](https://forum.qiime2.org/t/picrust-and-qiime2/4957/2 "2018-07-09T20:05:42Z")
To clarify, I have been able to find the commands/workflow needed to generate the correct files for PICRUSt using Qiime1. But I have not been able to find the commands/workflow needed to generate the correct files for PICRUSt using Qiime2.
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### Author:  [@ebolyen](https://forum.qiime2.org/u/ebolyen)
#### Post date: [July 10, 2018, 4:46pm UTC](https://forum.qiime2.org/t/picrust-and-qiime2/4957/3 "2018-07-10T16:46:42Z")
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### Author:  [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [July 11, 2018, 4:09pm UTC](https://forum.qiime2.org/t/picrust-and-qiime2/4957/4 "2018-07-11T16:09:05Z")
Hey there @ellen!
There are a [handful of topics that cover this on the forum already](https://forum.qiime2.org/search?q=picrust), have you had a chance to look at them? In particular, the summary listed [here](https://forum.qiime2.org/t/how-to-create-a-feature-table-with-qiime2-for-picrust-with-the-taxonomic-assignment/2526/8). The short guide provides a good high-level overview of the steps necessary to prepare data in QIIME 2 for analysis in picrust - let us know how it goes! :qiime2:
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### Author:  [@thermokarst](https://forum.qiime2.org/u/thermokarst)
#### Post date: [July 11, 2018, 4:09pm UTC](https://forum.qiime2.org/t/picrust-and-qiime2/4957/5 "2018-07-11T16:09:07Z")
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### Author:  [@system](https://forum.qiime2.org/u/system)
#### Post date: [August 11, 2018, 10:09pm UTC](https://forum.qiime2.org/t/picrust-and-qiime2/4957/6 "2018-08-11T22:09:12Z")
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