phylogenetic tree2

[ramezani62 (mohamad)]

I am following the qiime2 tutorial and trying to Generating a phylogenetic tree
qiime fragment-insertion sepp
–i-representative-sequences rep-seqs-deblur.qza
–i-reference-database sepp-refs-gg-13-8.qza
–p-threads 4
–o-tree insertion-tree.qza
–o-placements insertion-placements.qza
but there was a problem with this answer
Plugin error from fragment-insertion:

[Errno 12] Cannot allocate memory

Debug info has been saved to /tmp/qiime2-q2cli-err-0rj_p6nq.log
thanks

[SoilRotifer (Mike Robeson)]

Hi @ramezani62, welcome to !

This line:

@ramezani62

[Errno 12] Cannot allocate memory

Is informing you that your system does not have enough memory (RAM) to complete the task. You'll either have to use a system with more memory, or try using one of the other phylogeny inference approaches in QIIME 2, e.g. iqtree.

[ramezani62 (mohamad)]

Thank you very much for your help.