Percentage of reads classified at each taxon

[Liviacmg]

Thank you again!

I was facing an error but encountered the solution on this other topic:

" Feature IDs found in the table are missing in the metadata" but I have those features in metadata...

@SetaPark, You are doing the right thing with feature-table group but you are not grouping your sample metadata file correctly. As the error indicates, each sample ID must be unique, but all you have done is relabel the sample IDs of the original samples to match the group that they are being lumped into. You need to trim out duplicated lines from your sample metadata file so that each group is represented by a single line, and only group-wide metadata values are represented. So instead of thi…

Then, I runned the qiime taxa barplot like this (I had to remove the --i-taxonomy because it returned an error):

qiime taxa barplot
--i-table collapsed-table-l6.qza
--m-metadata-file sample-metadata.tsv
--o-visualization taxa-bar-plots.qzv

And I had to rerun taxa collapse for each level I wanted, and then generated the barplot for each of them and it worked.

And it appeared like this (this one at genus level):

Then, to know each taxon in percentage I did qiime feature-table relative-frequency and then transformed on csv with qiime metadata tabulate.