I am trying to import data in Qiime2 (2019.4). I get the error:
There was a problem importing manifest.csv:
manifest.csv is not a(n) PairedEndFastqManifestPhred33 file:
File referenced on line 2 could not be found ($PWD/sequence_files/SRR7629764_R1.fastq.gz).
I’ve checked the manifest.csv file, and even reduced it to one sample to trouble shoot. It contains only comma separated columns sample-id,absolute-filepath,direction
and the two sample lines are:
I’ve checked using TextWrangler and there is no other column, lines or formatting apparent for this file, so I cant work out why its not being recognised as the manifest file.
Any suggestions welcome!