Dear QIIME2 team,
I have a problem importing my PacBio fastq files into qiime2. I am trying with the manifest format, but whenever I run the command:
qiime tools import
–type ‘SampleData[SequencesWithQuality]’
–input-path /***/_test/se-64-manifest.gz
–output-path /***/_test/single-end-demux64.qza
–input-format SingleEndFastqManifestPhred64
I get a long error message, at the end saying:
ValueError: Decoded Phred score is out of range [0, 62].
An unexpected error has occurred:
Decoded Phred score is out of range [0, 62].
See above for debug info.
Any ideas how I can solve this problem? I would really love to try and analyze my data with qiime2. I am grateful for any support.