I am running ‘Deblur’ with Qiime2. Everything ran well but I find Deblur discarded lots of reads during preprocessing. Here is my case: I have multiple samples with around 20000 reads for each. After running Deblur, only less than 1000 reads were retained in each sample. I am really confused why so many reads were discarded by Deblur?
BTW, there is a special demand for me to know which reads go to which ASV. I am wondering if there is a way to find out this information?
Thanks in advance,