OTU-table with taxons and reads per sample, without clustering of taxons?

Interesting question, @Magreet. I believe your understanding of qiime taxa collapse is correct, but I don’t believe this functionality is currently available in QIIME 2.

Big-picture, I suspect what you’re trying to do is:

  • First, add taxonomic assignments to your original FeatureTable[Frequency].
  • Second, join that table with the Feature detail table of collapsed taxa, joining by taxon.

This would produce a long-form table with N ASVs for rows, and columns for feature count, taxonomy, taxa count, and maybe counts per sample.

If I’m understanding your desired outcome correctly, I think it will take a bit of data manipulation. There are a bunch of different ways to go about this, and Python, R, whatever language you prefer are probably the best approach. You could use Excel, but I suspect it would be rather painful (and I love spreadsheets!).

Here are a couple of somewhat-related topics you might want to check out for bread crumbs: 1, 2.

Hope that helps,
Chris :elephant:

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