Hi @ljy0676 ,
We have seen this reported just a couple times before — not sure why it occurs, but it sounds like sometimes q2-dada2 crashes between outputting the first and second files.
See here for some info and steps to troubleshoot:
Hello, I have read through a previous post with a similar topic name but didn't find how the issue was resolved. I have tried to run dada2 several times using the following:
qiime dada2 denoise-paired
--i-demultiplexed-seqs seqs.qza
--p-trim-left-f 0
--p-trim-left-r 0
--p-trunc-len-f 299
--p-trunc-len-r 256
--o-table table.qza
--o-representative-sequences rep_seqs.qza
--o-denoising-stats denoising_stats.qza
I have run it both with and without & on the end. Every time I have run…
Let us know if that helps!