# Only 2 two taxa from 18S taxa barplot

**URL:** https://forum.qiime2.org/t/only-2-two-taxa-from-18s-taxa-barplot/30891
**Category:** User Support
**Created:** [July 20, 2024, 4:30pm UTC](https://forum.qiime2.org/t/only-2-two-taxa-from-18s-taxa-barplot/30891 "2024-07-20T16:30:37Z")
**Posts on this page:** 1
**Showing post:** 15

<div class="post-metadata">

### Author: ![SoilRotifer](https://forum.qiime2.org/user_avatar/forum.qiime2.org/soilrotifer/32/21071_2.png) [@SoilRotifer](https://forum.qiime2.org/u/SoilRotifer)
#### Post date: [July 26, 2024, 9:35pm UTC](https://forum.qiime2.org/t/only-2-two-taxa-from-18s-taxa-barplot/30891/15 "2024-07-26T21:35:09Z")

</div>

HI @Namraj_Jaishi ,

I think the example premade classifiers might be too aggressive in their filtering. That is, any eukaryotic sequence less than 1400 bp is removed. This may be removing too many valid reference sequences. I am thinking that you should make your own classifier as outlined [here](https://forum.qiime2.org/t/using-silva-138-pre-trained-classifier/30857/5). Then let us know how it works.

---

_[View the full topic](https://forum.qiime2.org/t/only-2-two-taxa-from-18s-taxa-barplot/30891)._