Now I am in the taxonomy assignment step. Do you have any tips or link to tutorials in which classify-consensus-vsearch is used?

Hi @alexcussigh0,

Check out our RESCRIPt 12S db tutorial. It's just a simple example of what you can do, but it should help you get started...

Although it means re-downloading everything again, and potentially in batches by taxonomic group. But everything will be formatted properly.

The notebook specifically looks for records of 12S reads, but you can perform a separate search that downloads only genome records and then use feature-classifier extract-reads to extract the ampicon region from those genomes. Then you can merge with the other batches of data.

Anyway, I just wanted to provide another option that you can try running in parallel.

-Mike

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