# Not getting anything from the .qzv file **URL:** https://forum.qiime2.org/t/not-getting-anything-from-the-qzv-file/31946 **Category:** User Support **Created:** [November 21, 2024, 7:14am UTC](https://forum.qiime2.org/t/not-getting-anything-from-the-qzv-file/31946 "2024-11-21T07:14:19Z") **Posts on this page:** 1 **Showing post:** 4
### Author: ![colinbrislawn](https://forum.qiime2.org/user_avatar/forum.qiime2.org/colinbrislawn/32/6221_2.png) [@colinbrislawn](https://forum.qiime2.org/u/colinbrislawn) #### Post date: [November 21, 2024, 11:42pm UTC](https://forum.qiime2.org/t/not-getting-anything-from-the-qzv-file/31946/4 "2024-11-21T23:42:59Z")
Yes! These are probably Illumina's new 'binned' quality scores. They can cause issue with DADA2, _sometimes_. > [@NovaSeq and Dada2 incompatibility.](https://forum.qiime2.org/t/novaseq-and-dada2-incompatibility/25865): > > Dear All, I have been analysing NovaSeq 6000 16s rRNA V4-V5 data for the past few months. This is my first experience with NGS data analysis. As a novice, I was sideswiped by the fact that NovaSeq results were very different due to their binned quality scores. From multiple posts, the consensus is that Dada2 is not appropriate for NovaSeq sequences unless you can enforce monotonicity. This leads me to the question, has Qiime2 implemented a way to run the Dada2 denoising algorithm by enforcing … I would continue with cautious optimism and see how it goes! > which feels like all the sequences are the same That would be an issue, and you could see that during denoising or during taxonomy assignment. Or maybe it's fine. --- _[View the full topic](https://forum.qiime2.org/t/not-getting-anything-from-the-qzv-file/31946)._