Normalization use

it is not necessary to do both — if you've read otherwise in our documentation, please tell us where so we can get that fixed :wink:

Absolutely.

That normalization is not adequate for some analyses (e.g., alpha diversity comparisons require normalization for uneven sequencing depth between samples — currently we recommend rarefying to correct for that)

Any analysis in QIIME 2 (at least for "core" plugins) that requires normalization has that normalization built in — e.g., rarefying is performed automatically for alpha and beta diversity analyses. Rarefied tables should not be used for downstream analyses, e.g., that involve converting to relative abundance.

Did you have a particular step in mind?

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