I am implementing q2-itsxpress in the analysis of ITS sequences.
After running below code,
qiime itsxpress trim-pair-output-unmerged
–p-region ITS2 --p-taxa F --p-threads 2
I got the following error message,
No reads passed the filter. trunc_len_f (0) or trunc_len_r (0) may be individually longer than read lengths, or trunc_len_f + trunc_len_r may be shorter than the length of the amplicon + 20 nucleotides (the length of the overlap). Alternatively, other arguments (such as max_ee or trunc_q) may be preventing reads from passing the filter.
I have tried to visualize the data using demux summarize on trimmed reads and failed with the following error message.
Plugin error from demux:
Cannot describe a DataFrame without columns
Debug info has been saved to /tmp/qiime2-q2cli-err-iku7o_p7.log
These are maybe two threads, but might be linked to the q2-itsxpress output file!
I used qiime2-2018.11
Is there different output formats from q2-itsxpress that I should specify or pass to dada2 and qiime demux summarize? I
Thank you for inputs.