Dear @jwdebelius,
Thanks sincerely. I think I made some conceptional mistake of fragment insertion SEEP and fragment insertion filter feature.
My understanding to fragment insertion is to re-organize my tree by a reference tree. The intension should be the prevention of exaggerating the difference during beta diversity analysis. But what's the role of the feature table?
I just wandering that the function fragment-insertion filter-features might remove some of the feature from the original table, but when I checked, it seemed nothing changed.
Latter, when I followed the instruction of PICRUSt2 Swapping in SEPP for read placement with q2 picrust2 pipeline, I inputed the filtered_table.qza and insertion-tree.qza and I got:
Standard error of failed command:
"Error: None of the reference ids within the function abundance table are found within the input tree. This can occur when malformed or mismatched custom reference files are used.
Execution halted
I guess I did something wrong in the fragment insertion procedure, do you have any idea about that?
Thank you very much!!