New into QIIME2 and need help importing Data

[Nicholas_Bokulich]

Hi @Vixer,

@Vixer

Hello, I really new in bioinformatics and qiime2

Welcome! If you have not done so already, please see our getting started guide and tutorials to help get you started with QIIME2.

@Vixer

First, the paired-end raw data I reciebed has the filename “Sam1-16_S29_L002_R1_001.fastq.gz” and “Sam1-16_S29_L002_R1_001.fastq.gz” but i dont seem to have an barcode.fasta.qz archive. So I cant do the EMP protocol multiplexed paired-end fastq.

QIIME2 supports importing raw data in several different formats. It sounds like your data are per-sample CASAVA1.8 format. They do not have associated barcode files, but that's okay — each sample has already been demultiplexed into its own file.

Alternatively, you might have barcodes in the reads (this is not clear from your description). If that's the case, please see this community tutorial.

I hope that helps!