# NaNs in q2-perc-norm output

**URL:** https://forum.qiime2.org/t/nans-in-q2-perc-norm-output/9172
**Category:** Library Support
**Created:** [April 8, 2019, 3:55pm UTC](https://forum.qiime2.org/t/nans-in-q2-perc-norm-output/9172 "2019-04-08T15:55:57Z")
**Posts on this page:** 1
**Showing post:** 5

<div class="post-metadata">

### Author: ![cduvallet](https://forum.qiime2.org/user_avatar/forum.qiime2.org/cduvallet/32/1082_2.png) [@cduvallet](https://forum.qiime2.org/u/cduvallet)
#### Post date: [May 29, 2019, 6:44pm UTC](https://forum.qiime2.org/t/nans-in-q2-perc-norm-output/9172/5 "2019-05-29T18:44:35Z")

</div>

Sorry to hear there are still issues, @prince. I'm currently traveling, so apologies for the delay in responding. If you'd like faster responses, it's helpful to provide exactly what data you used and commands you ran, so I can quickly try to reproduce the error on my computer. You should also include what versions of qiime2 and the plugin you are using.

I just ran the following command using the crc\_metadata.tsv and crc\_relative.qza files provided in @cdiener's [workshop repo](https://github.com/Gibbons-Lab/ccmb_workshop) and did not get any errors. I'm using the 2019.1 version of q2-perc-norm on the 2019.1 version of qiime2.

```bash
qiime perc-norm percentile-normalize \
	--i-table crc_relative.qza \
	--m-metadata-file crc_metadata.tsv \
	--m-metadata-column disease_state \
	--m-batch-file crc_metadata.tsv \
	--m-batch-column study \
	--o-perc-norm-table percentile_normalized.qza

```

> [@prince](#):
>
> How bad is using --p-otu-thresh 0.0 ?

Lowering the OTU presence threshold to 0 is not really recommended. We discussed this on a previous post: [Q2-perc-norm - reduction in taxa](https://forum.qiime2.org/t/q2-perc-norm-reduction-in-taxa/7192)

---

_[View the full topic](https://forum.qiime2.org/t/nans-in-q2-perc-norm-output/9172)._