# Naive Bayes classifier for the 515F/806R region

**URL:** https://forum.qiime2.org/t/naive-bayes-classifier-for-the-515f-806r-region/23986
**Category:** User Support
**Tags:** feature-classifier
**Created:** [August 25, 2022, 1:11pm UTC](https://forum.qiime2.org/t/naive-bayes-classifier-for-the-515f-806r-region/23986 "2022-08-25T13:11:21Z")
**Posts on this page:** 4
**Page:** 1

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### Author: ![Antonio\_Louvado](https://forum.qiime2.org/user_avatar/forum.qiime2.org/antonio_louvado/32/11758_2.png) [@Antonio\_Louvado](https://forum.qiime2.org/u/Antonio_Louvado)
#### Post date: [August 25, 2022, 1:11pm UTC](https://forum.qiime2.org/t/naive-bayes-classifier-for-the-515f-806r-region/23986/1 "2022-08-25T13:11:22Z")

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Hi,

I used the Naive Bayes classifier trained on primers 515F/806R available on [Data resources — QIIME 2 2022.2.0 documentation](https://docs.qiime2.org/2022.2/data-resources/). I recently notice that in the earth microbiome protocol their are two versions of primer for the 515F/806R regions.

Updated sequences: 515F (Parada)–806R (Apprill), forward-barcoded:  
FWD:GTGYCAGCMGCCGCGGTAA; REV:GGACTACNVGGGTWTCTAAT  
Original sequences: 515F (Caporaso)–806R (Caporaso), reverse-barcoded:  
FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT

After a thorough search on qiime forum and internet I did not find information regarding which primer set was used to train the classifier. Had you inform if the this classifier used the updated or original sequences?\

Best regards,  
Antonio

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### Author: ![SoilRotifer](https://forum.qiime2.org/user_avatar/forum.qiime2.org/soilrotifer/32/21071_2.png) [@SoilRotifer](https://forum.qiime2.org/u/SoilRotifer)
#### Post date: [August 25, 2022, 1:43pm UTC](https://forum.qiime2.org/t/naive-bayes-classifier-for-the-515f-806r-region/23986/2 "2022-08-25T13:43:42Z")

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Hi @Antonio_Louvado, welcome to :qiime2:!

You can determine this by looking through the provenance information of the `silva-138-99-515-806-nb-classifier.qza` file. That is, simply drag-drop this file into [QIIME 2 View](https://view.qiime2.org/), then click on the provenance tab. From here you can click on each item in the provenance graph to see what parameters were used for each command. Like this:

![silva-v4-provgraph](https://forum.qiime2.org/images/transparent.png)

Here you can see, the primer sequence that where used. It looks like the original primer sequences where used.

_Personal note: The only difference between these two primer sets is the additional IUPAC ambiguity bases in the "updated" primer sequences. When I make the classifier I use the "updated" primer set, as they are more "universal" than the original sequences. Which means better success in finding and searching for your amplicons._

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### Author: ![Antonio\_Louvado](https://forum.qiime2.org/user_avatar/forum.qiime2.org/antonio_louvado/32/11758_2.png) [@Antonio\_Louvado](https://forum.qiime2.org/u/Antonio_Louvado)
#### Post date: [August 25, 2022, 3:01pm UTC](https://forum.qiime2.org/t/naive-bayes-classifier-for-the-515f-806r-region/23986/3 "2022-08-25T15:01:42Z")

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Thanks very much for the information.

Antonio

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### Author: ![system](https://forum-qiime2-org.s3.dualstack.us-west-2.amazonaws.com/original/3X/2/1/21af5fe23cb6f4579467c66a9ed94e55274ca7bd.svg) [@system](https://forum.qiime2.org/u/system)
#### Post date: [September 25, 2022, 9:02pm UTC](https://forum.qiime2.org/t/naive-bayes-classifier-for-the-515f-806r-region/23986/4 "2022-09-25T21:02:39Z")

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