# Mycobiome\_ITS\_Remove primers & barcode sequence

**URL:** https://forum.qiime2.org/t/mycobiome-its-remove-primers-barcode-sequence/7515
**Category:** General Discussion
**Created:** [December 18, 2018, 6:26pm UTC](https://forum.qiime2.org/t/mycobiome-its-remove-primers-barcode-sequence/7515 "2018-12-18T18:26:26Z")
**Posts on this page:** 1
**Showing post:** 2

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### Author: ![Nicholas\_Bokulich](https://forum.qiime2.org/user_avatar/forum.qiime2.org/nicholas_bokulich/32/19937_2.png) [@Nicholas\_Bokulich](https://forum.qiime2.org/u/Nicholas_Bokulich)
#### Post date: [December 19, 2018, 1:53pm UTC](https://forum.qiime2.org/t/mycobiome-its-remove-primers-barcode-sequence/7515/2 "2018-12-19T13:53:26Z")

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You can use q2-cutadapt for this. See this tutorial for an example with fungal ITS data:

> [@Fungal ITS analysis tutorial](https://forum.qiime2.org/t/fungal-its-analysis-tutorial/7351):
>
> Fungal ITS analysis, mock communities, and more fun NOTE: This tutorial was written in a QIIME 2 2018.11 environment. It is not guaranteed to work with earlier or later versions of QIIME 2. This tutorial was compiled as a working exercise for a [QIIME 2 workshop](https://workshops.qiime2.org/microbiome-bioinformatics-qiime-2-2018-12-12/) in December 2018, and does not represent the only possible fungal ITS workflow with QIIME 2, or even a benchmarked protocol recommendation. See other tutorials, e.g., the [q2-itsxpress tutorial](https://forum.qiime2.org/t/q2-itsxpress-a-tutorial-on-a-qiime-2-plugin-to-trim-its-sequences/5780) for other fungal ITS analysis options in QII…

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